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Thus, the CpG dinucleotide is essential for normal erythropoiesis and this study illustrates how a single methylated CpG can directly affect transcription factor binding and cellular regulation

The iron can cycle between two oxidation states, ferric ion [Fe(III) or Fe 3+ ] and ferrous ion [Fe(II) or Fe 2+ ], allowing it to act as an electron donor and acceptor [23]

These molecular and cellular events lead to a severe inflammatory state in a broad range of tissues, causing irreversible damage

Protein concentration of the supernatant was quantified using a Lowry assay and each sample was aliquoted and diluted to a total of 100 l at 5 g/l